Java Native Access（JNA）是用于处理本机库的出色项目，并且通过Advapi32Util和Advapi32支持平台库（platform.jar）中的Windows注册表。

import com.sun.jna.platform.win32.Advapi32Util;
import com.sun.jna.platform.win32.WinReg;

public class WindowsRegistrySnippet {
    public static void main(String[] args) {
        // Read a string
        String productName = Advapi32Util.registryGetStringValue(
            WinReg.HKEY_LOCAL_MACHINE, "SOFTWARE\\Microsoft\\Windows NT\\CurrentVersion", "ProductName");
        System.out.printf("Product Name: %s\n", productName);

        // Read an int (& 0xFFFFFFFFL for large unsigned int)
        int timeout = Advapi32Util.registryGetIntValue(
            WinReg.HKEY_LOCAL_MACHINE, "SOFTWARE\\Microsoft\\Windows NT\\CurrentVersion\\Windows", "ShutdownWarningDialogTimeout");
        System.out.printf("Shutdown Warning Dialog Timeout: %d (%d as unsigned long)\n", timeout, timeout & 0xFFFFFFFFL);

        // Create a key and write a string
        Advapi32Util.registryCreateKey(WinReg.HKEY_CURRENT_USER, "SOFTWARE\\StackOverflow");
        Advapi32Util.registrySetStringValue(WinReg.HKEY_CURRENT_USER, "SOFTWARE\\StackOverflow", "url", "http://stackoverflow.com/a/6287763/277307");

        // Delete a key
        Advapi32Util.registryDeleteKey(WinReg.HKEY_CURRENT_USER, "SOFTWARE\\StackOverflow");
    }
}

我增加了David最初发布的Pure java代码，以允许从64位JVM访问注册表的32位部分，反之亦然。我认为没有其他答案可以解决这个问题。

这里是：

/**
 * Pure Java Windows Registry access.
 * Modified by petrucio@stackoverflow(828681) to add support for
 * reading (and writing but not creating/deleting keys) the 32-bits
 * registry view from a 64-bits JVM (KEY_WOW64_32KEY)
 * and 64-bits view from a 32-bits JVM (KEY_WOW64_64KEY).
 *****************************************************************************/

import java.lang.reflect.InvocationTargetException;
import java.lang.reflect.Method;
import java.util.HashMap;
import java.util.Map;
import java.util.ArrayList;
import java.util.List;
import java.util.prefs.Preferences;

public class WinRegistry {
  public static final int HKEY_CURRENT_USER = 0x80000001;
  public static final int HKEY_LOCAL_MACHINE = 0x80000002;
  public static final int REG_SUCCESS = 0;
  public static final int REG_NOTFOUND = 2;
  public static final int REG_ACCESSDENIED = 5;

  public static final int KEY_WOW64_32KEY = 0x0200;
  public static final int KEY_WOW64_64KEY = 0x0100;

  private static final int KEY_ALL_ACCESS = 0xf003f;
  private static final int KEY_READ = 0x20019;
  private static Preferences userRoot = Preferences.userRoot();
  private static Preferences systemRoot = Preferences.systemRoot();
  private static Class<? extends Preferences> userClass = userRoot.getClass();
  private static Method regOpenKey = null;
  private static Method regCloseKey = null;
  private static Method regQueryValueEx = null;
  private static Method regEnumValue = null;
  private static Method regQueryInfoKey = null;
  private static Method regEnumKeyEx = null;
  private static Method regCreateKeyEx = null;
  private static Method regSetValueEx = null;
  private static Method regDeleteKey = null;
  private static Method regDeleteValue = null;

  static {
    try {
      regOpenKey     = userClass.getDeclaredMethod("WindowsRegOpenKey",     new Class[] { int.class, byte[].class, int.class });
      regOpenKey.setAccessible(true);
      regCloseKey    = userClass.getDeclaredMethod("WindowsRegCloseKey",    new Class[] { int.class });
      regCloseKey.setAccessible(true);
      regQueryValueEx= userClass.getDeclaredMethod("WindowsRegQueryValueEx",new Class[] { int.class, byte[].class });
      regQueryValueEx.setAccessible(true);
      regEnumValue   = userClass.getDeclaredMethod("WindowsRegEnumValue",   new Class[] { int.class, int.class, int.class });
      regEnumValue.setAccessible(true);
      regQueryInfoKey=userClass.getDeclaredMethod("WindowsRegQueryInfoKey1",new Class[] { int.class });
      regQueryInfoKey.setAccessible(true);
      regEnumKeyEx   = userClass.getDeclaredMethod("WindowsRegEnumKeyEx",   new Class[] { int.class, int.class, int.class });  
      regEnumKeyEx.setAccessible(true);
      regCreateKeyEx = userClass.getDeclaredMethod("WindowsRegCreateKeyEx", new Class[] { int.class, byte[].class });
      regCreateKeyEx.setAccessible(true);  
      regSetValueEx  = userClass.getDeclaredMethod("WindowsRegSetValueEx",  new Class[] { int.class, byte[].class, byte[].class });  
      regSetValueEx.setAccessible(true); 
      regDeleteValue = userClass.getDeclaredMethod("WindowsRegDeleteValue", new Class[] { int.class, byte[].class });  
      regDeleteValue.setAccessible(true); 
      regDeleteKey   = userClass.getDeclaredMethod("WindowsRegDeleteKey",   new Class[] { int.class, byte[].class });  
      regDeleteKey.setAccessible(true); 
    }
    catch (Exception e) {
      e.printStackTrace();
    }
  }

  private WinRegistry() {  }

  /**
   * Read a value from key and value name
   * @param hkey   HKEY_CURRENT_USER/HKEY_LOCAL_MACHINE
   * @param key
   * @param valueName
   * @param wow64  0 for standard registry access (32-bits for 32-bit app, 64-bits for 64-bits app)
   *               or KEY_WOW64_32KEY to force access to 32-bit registry view,
   *               or KEY_WOW64_64KEY to force access to 64-bit registry view
   * @return the value
   * @throws IllegalArgumentException
   * @throws IllegalAccessException
   * @throws InvocationTargetException
   */
  public static String readString(int hkey, String key, String valueName, int wow64) 
    throws IllegalArgumentException, IllegalAccessException,
    InvocationTargetException 
  {
    if (hkey == HKEY_LOCAL_MACHINE) {
      return readString(systemRoot, hkey, key, valueName, wow64);
    }
    else if (hkey == HKEY_CURRENT_USER) {
      return readString(userRoot, hkey, key, valueName, wow64);
    }
    else {
      throw new IllegalArgumentException("hkey=" + hkey);
    }
  }

  /**
   * Read value(s) and value name(s) form given key 
   * @param hkey  HKEY_CURRENT_USER/HKEY_LOCAL_MACHINE
   * @param key
   * @param wow64  0 for standard registry access (32-bits for 32-bit app, 64-bits for 64-bits app)
   *               or KEY_WOW64_32KEY to force access to 32-bit registry view,
   *               or KEY_WOW64_64KEY to force access to 64-bit registry view
   * @return the value name(s) plus the value(s)
   * @throws IllegalArgumentException
   * @throws IllegalAccessException
   * @throws InvocationTargetException
   */
  public static Map<String, String> readStringValues(int hkey, String key, int wow64) 
    throws IllegalArgumentException, IllegalAccessException, InvocationTargetException 
  {
    if (hkey == HKEY_LOCAL_MACHINE) {
      return readStringValues(systemRoot, hkey, key, wow64);
    }
    else if (hkey == HKEY_CURRENT_USER) {
      return readStringValues(userRoot, hkey, key, wow64);
    }
    else {
      throw new IllegalArgumentException("hkey=" + hkey);
    }
  }

  /**
   * Read the value name(s) from a given key
   * @param hkey  HKEY_CURRENT_USER/HKEY_LOCAL_MACHINE
   * @param key
   * @param wow64  0 for standard registry access (32-bits for 32-bit app, 64-bits for 64-bits app)
   *               or KEY_WOW64_32KEY to force access to 32-bit registry view,
   *               or KEY_WOW64_64KEY to force access to 64-bit registry view
   * @return the value name(s)
   * @throws IllegalArgumentException
   * @throws IllegalAccessException
   * @throws InvocationTargetException
   */
  public static List<String> readStringSubKeys(int hkey, String key, int wow64) 
    throws IllegalArgumentException, IllegalAccessException, InvocationTargetException 
  {
    if (hkey == HKEY_LOCAL_MACHINE) {
      return readStringSubKeys(systemRoot, hkey, key, wow64);
    }
    else if (hkey == HKEY_CURRENT_USER) {
      return readStringSubKeys(userRoot, hkey, key, wow64);
    }
    else {
      throw new IllegalArgumentException("hkey=" + hkey);
    }
  }

  /**
   * Create a key
   * @param hkey  HKEY_CURRENT_USER/HKEY_LOCAL_MACHINE
   * @param key
   * @throws IllegalArgumentException
   * @throws IllegalAccessException
   * @throws InvocationTargetException
   */
  public static void createKey(int hkey, String key) 
    throws IllegalArgumentException, IllegalAccessException, InvocationTargetException 
  {
    int [] ret;
    if (hkey == HKEY_LOCAL_MACHINE) {
      ret = createKey(systemRoot, hkey, key);
      regCloseKey.invoke(systemRoot, new Object[] { new Integer(ret[0]) });
    }
    else if (hkey == HKEY_CURRENT_USER) {
      ret = createKey(userRoot, hkey, key);
      regCloseKey.invoke(userRoot, new Object[] { new Integer(ret[0]) });
    }
    else {
      throw new IllegalArgumentException("hkey=" + hkey);
    }
    if (ret[1] != REG_SUCCESS) {
      throw new IllegalArgumentException("rc=" + ret[1] + "  key=" + key);
    }
  }

  /**
   * Write a value in a given key/value name
   * @param hkey
   * @param key
   * @param valueName
   * @param value
   * @param wow64  0 for standard registry access (32-bits for 32-bit app, 64-bits for 64-bits app)
   *               or KEY_WOW64_32KEY to force access to 32-bit registry view,
   *               or KEY_WOW64_64KEY to force access to 64-bit registry view
   * @throws IllegalArgumentException
   * @throws IllegalAccessException
   * @throws InvocationTargetException
   */
  public static void writeStringValue
    (int hkey, String key, String valueName, String value, int wow64) 
    throws IllegalArgumentException, IllegalAccessException, InvocationTargetException 
  {
    if (hkey == HKEY_LOCAL_MACHINE) {
      writeStringValue(systemRoot, hkey, key, valueName, value, wow64);
    }
    else if (hkey == HKEY_CURRENT_USER) {
      writeStringValue(userRoot, hkey, key, valueName, value, wow64);
    }
    else {
      throw new IllegalArgumentException("hkey=" + hkey);
    }
  }

  /**
   * Delete a given key
   * @param hkey
   * @param key
   * @throws IllegalArgumentException
   * @throws IllegalAccessException
   * @throws InvocationTargetException
   */
  public static void deleteKey(int hkey, String key) 
    throws IllegalArgumentException, IllegalAccessException, InvocationTargetException 
  {
    int rc = -1;
    if (hkey == HKEY_LOCAL_MACHINE) {
      rc = deleteKey(systemRoot, hkey, key);
    }
    else if (hkey == HKEY_CURRENT_USER) {
      rc = deleteKey(userRoot, hkey, key);
    }
    if (rc != REG_SUCCESS) {
      throw new IllegalArgumentException("rc=" + rc + "  key=" + key);
    }
  }

  /**
   * delete a value from a given key/value name
   * @param hkey
   * @param key
   * @param value
   * @param wow64  0 for standard registry access (32-bits for 32-bit app, 64-bits for 64-bits app)
   *               or KEY_WOW64_32KEY to force access to 32-bit registry view,
   *               or KEY_WOW64_64KEY to force access to 64-bit registry view
   * @throws IllegalArgumentException
   * @throws IllegalAccessException
   * @throws InvocationTargetException
   */
  public static void deleteValue(int hkey, String key, String value, int wow64) 
    throws IllegalArgumentException, IllegalAccessException, InvocationTargetException 
  {
    int rc = -1;
    if (hkey == HKEY_LOCAL_MACHINE) {
      rc = deleteValue(systemRoot, hkey, key, value, wow64);
    }
    else if (hkey == HKEY_CURRENT_USER) {
      rc = deleteValue(userRoot, hkey, key, value, wow64);
    }
    if (rc != REG_SUCCESS) {
      throw new IllegalArgumentException("rc=" + rc + "  key=" + key + "  value=" + value);
    }
  }

  //========================================================================
  private static int deleteValue(Preferences root, int hkey, String key, String value, int wow64)
    throws IllegalArgumentException, IllegalAccessException, InvocationTargetException 
  {
    int[] handles = (int[]) regOpenKey.invoke(root, new Object[] {
        new Integer(hkey), toCstr(key), new Integer(KEY_ALL_ACCESS | wow64)
    });
    if (handles[1] != REG_SUCCESS) {
      return handles[1];  // can be REG_NOTFOUND, REG_ACCESSDENIED
    }
    int rc =((Integer) regDeleteValue.invoke(root, new Object[] { 
          new Integer(handles[0]), toCstr(value) 
          })).intValue();
    regCloseKey.invoke(root, new Object[] { new Integer(handles[0]) });
    return rc;
  }

  //========================================================================
  private static int deleteKey(Preferences root, int hkey, String key) 
    throws IllegalArgumentException, IllegalAccessException, InvocationTargetException 
  {
    int rc =((Integer) regDeleteKey.invoke(root, new Object[] {
        new Integer(hkey), toCstr(key)
    })).intValue();
    return rc;  // can REG_NOTFOUND, REG_ACCESSDENIED, REG_SUCCESS
  }

  //========================================================================
  private static String readString(Preferences root, int hkey, String key, String value, int wow64)
    throws IllegalArgumentException, IllegalAccessException, InvocationTargetException 
  {
    int[] handles = (int[]) regOpenKey.invoke(root, new Object[] {
        new Integer(hkey), toCstr(key), new Integer(KEY_READ | wow64)
    });
    if (handles[1] != REG_SUCCESS) {
      return null; 
    }
    byte[] valb = (byte[]) regQueryValueEx.invoke(root, new Object[] {
        new Integer(handles[0]), toCstr(value)
    });
    regCloseKey.invoke(root, new Object[] { new Integer(handles[0]) });
    return (valb != null ? new String(valb).trim() : null);
  }

  //========================================================================
  private static Map<String,String> readStringValues(Preferences root, int hkey, String key, int wow64)
    throws IllegalArgumentException, IllegalAccessException, InvocationTargetException 
  {
    HashMap<String, String> results = new HashMap<String,String>();
    int[] handles = (int[]) regOpenKey.invoke(root, new Object[] {
        new Integer(hkey), toCstr(key), new Integer(KEY_READ | wow64)
    });
    if (handles[1] != REG_SUCCESS) {
      return null;
    }
    int[] info = (int[]) regQueryInfoKey.invoke(root, new Object[] {
        new Integer(handles[0])
    });

    int count  = info[2]; // count  
    int maxlen = info[3]; // value length max
    for(int index=0; index<count; index++)  {
      byte[] name = (byte[]) regEnumValue.invoke(root, new Object[] {
          new Integer(handles[0]), new Integer(index), new Integer(maxlen + 1)
      });
      String value = readString(hkey, key, new String(name), wow64);
      results.put(new String(name).trim(), value);
    }
    regCloseKey.invoke(root, new Object[] { new Integer(handles[0]) });
    return results;
  }

  //========================================================================
  private static List<String> readStringSubKeys(Preferences root, int hkey, String key, int wow64)
    throws IllegalArgumentException, IllegalAccessException, InvocationTargetException 
  {
    List<String> results = new ArrayList<String>();
    int[] handles = (int[]) regOpenKey.invoke(root, new Object[] {
        new Integer(hkey), toCstr(key), new Integer(KEY_READ | wow64) 
        });
    if (handles[1] != REG_SUCCESS) {
      return null;
    }
    int[] info = (int[]) regQueryInfoKey.invoke(root, new Object[] {
        new Integer(handles[0])
    });

    int count  = info[0]; // Fix: info[2] was being used here with wrong results. Suggested by davenpcj, confirmed by Petrucio
    int maxlen = info[3]; // value length max
    for(int index=0; index<count; index++)  {
      byte[] name = (byte[]) regEnumKeyEx.invoke(root, new Object[] {
          new Integer(handles[0]), new Integer(index), new Integer(maxlen + 1)
          });
      results.add(new String(name).trim());
    }
    regCloseKey.invoke(root, new Object[] { new Integer(handles[0]) });
    return results;
  }

  //========================================================================
  private static int [] createKey(Preferences root, int hkey, String key)
    throws IllegalArgumentException, IllegalAccessException, InvocationTargetException 
  {
    return (int[]) regCreateKeyEx.invoke(root, new Object[] {
      new Integer(hkey), toCstr(key)
    });
  }

  //========================================================================
  private static void writeStringValue(Preferences root, int hkey, String key, String valueName, String value, int wow64)
    throws IllegalArgumentException, IllegalAccessException, InvocationTargetException 
  {
    int[] handles = (int[]) regOpenKey.invoke(root, new Object[] {
        new Integer(hkey), toCstr(key), new Integer(KEY_ALL_ACCESS | wow64)
    });
    regSetValueEx.invoke(root, new Object[] { 
          new Integer(handles[0]), toCstr(valueName), toCstr(value) 
          }); 
    regCloseKey.invoke(root, new Object[] { new Integer(handles[0]) });
  }

  //========================================================================
  // utility
  private static byte[] toCstr(String str) {
    byte[] result = new byte[str.length() + 1];

    for (int i = 0; i < str.length(); i++) {
      result[i] = (byte) str.charAt(i);
    }
    result[str.length()] = 0;
    return result;
  }
}