如果要在virtualenv下运行Python子进程，可以通过使用位于virtualenv的/ bin /目录中的Python解释器运行脚本来实现：

# Path to a Python interpreter that runs any Python script
# under the virtualenv /path/to/virtualenv/
python_bin = "/path/to/virtualenv/bin/python"

# Path to the script that must run under the virtualenv
script_file = "must/run/under/virtualenv/script.py"

subprocess.Popen([python_bin, script_file])

但是，如果要在当前的Python解释器下而不是子进程下激活virtualenv，则可以使用以下activate_this.py脚本：

# Doing execfile() on this file will alter the current interpreter's
# environment so you can import libraries in the virtualenv
activate_this_file = "/path/to/virtualenv/bin/activate_this.py"

execfile(activate_this_file, dict(__file__=activate_this_file))