你可以做：

DataGridViewRow row = (DataGridViewRow)yourDataGridView.Rows[0].Clone();
row.Cells[0].Value = "XYZ";
row.Cells[1].Value = 50.2;
yourDataGridView.Rows.Add(row);

要么：

DataGridViewRow row = (DataGridViewRow)yourDataGridView.Rows[0].Clone();
row.Cells["Column2"].Value = "XYZ";
row.Cells["Column6"].Value = 50.2;
yourDataGridView.Rows.Add(row);

另一种方式：

this.dataGridView1.Rows.Add("five", "six", "seven","eight");
this.dataGridView1.Rows.Insert(0, "one", "two", "three", "four");

来自：http :
//msdn.microsoft.com/zh-
cn/library/system.windows.forms.datagridview.rows.aspx